Determining the effect of pretreatments on freeze resistance and survival of cryopreserved temperate fruit tree dormant buds

Freeze resistance is critical to successful dormant bud (DB) cryopreservation, and affected by genotype, environmental conditions, dormancy phase processing techniques. Pretreatment induced freeze may contribute more efficient protocols for cryopreserving DB. Differential thermal analysis (DTA) was used quantify the effects of cryopreservation pretreatments on budwood. Low temperature exotherm (LTE) profiles created DTA could rapidly identify that are contributing increased in tree fruit species. In this study, help elucidate varying (sucrose, desiccation their combination) peach, a model crop physiology has shown little cryosurvival using DB method past. Post recovery trials an antimicrobial forced development (AFBD) protocol evaluated ability selected pretreatments, improved based DTA, improve budwood various deciduous nut Precryogenic exposure sucrose solution (5.0 M, 96 h), 30% moisture content (MC) combination tested efficacy improving postcryogenic viability apricot, sweet cherry, walnut, black English apple, pear. Among different tested, MC had greatest impact increasing across most species walnut. Gradual reduction (from 40 25%) levels peach (R2=0.95) some outcomes (leaf, shoot swell), however, not correlated with equal cryorecovery as severe cracking observed. Overall, our approach linking preconditioning treatments establish species-specific number important temperate woody crops which be recovered complete plants coupling AFBD plant tissue culture.